\u0110i\u1ec7n di DNA c\u1ee7a agarose y\u00eau c\u1ea7u m\u1ed9t b\u01b0\u1edbc nhu\u1ed9m m\u00e0u \u0111\u1ec3 h\u00ecnh dung c\u00e1c \u0111o\u1ea1n DNA.\u00a0\u0110i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n m\u1ed9t c\u00e1ch an to\u00e0n b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng dung d\u1ecbch xanh metylen.\u00a0Xanh methylene li\u00ean k\u1ebft v\u1edbi DNA t\u00edch \u0111i\u1ec7n \u00e2m l\u00e0m cho DNA c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y d\u01b0\u1edbi d\u1ea1ng c\u00e1c d\u1ea3i m\u00e0u xanh lam trong agarose sau khi \u0111i\u1ec7n di.<\/p>\n
Dung d\u1ecbch xanh metylen \u0111\u01b0\u1ee3c cung c\u1ea5p \u1edf d\u1ea1ng \u0111\u1eadm \u0111\u1eb7c g\u1ea5p 10 l\u1ea7n.\u00a0\u0110\u1ec3 s\u1eed d\u1ee5ng, pha lo\u00e3ng dung d\u1ecbch \u0111\u1eadm \u0111\u1eb7c 10x b\u1eb1ng c\u00e1ch tr\u1ed9n m\u1ed9t ph\u1ea7n xanh methylene v\u1edbi ch\u00edn ph\u1ea7n n\u01b0\u1edbc (V\u00ed d\u1ee5: 100 ml dung d\u1ecbch \u0111\u1eadm \u0111\u1eb7c 10x \u0111\u01b0\u1ee3c th\u00eam v\u00e0o 900 ml n\u01b0\u1edbc).<\/p>\n
Sau \u0111\u00f3, xanh methylene 1x c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 nhu\u1ed9m c\u00e1c d\u1ea3i DNA trong gel agarose.\u00a0\u0110\u1eb7t gel Agarose c\u1ee7a b\u1ea1n v\u00e0o khay nhu\u1ed9m gel s\u1ea1ch, nh\u1ecf.\u00a0\u0110\u1ed5 v\u1eeba \u0111\u1ee7 1x thu\u1ed1c nhu\u1ed9m xanh metylen v\u00e0o m\u1ed9t khay th\u00edch h\u1ee3p \u0111\u1ec3 ph\u1ee7 gel agarose.\u00a0\u0110\u1ec3 y\u00ean cho \u0111\u1ebfn khi c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y c\u00e1c d\u1ea3i DNA trong agarose.\u00a0Qu\u00e1 tr\u00ecnh n\u00e0y c\u00f3 th\u1ec3 m\u1ea5t t\u1edbi 16 gi\u1edd (qua \u0111\u00eam).\u00a0Sau khi nhu\u1ed9m xong, r\u1eeda gel agarose b\u1eb1ng n\u01b0\u1edbc s\u1ea1ch.\u00a0\u0110\u1ed9 ph\u00e2n gi\u1ea3i c\u1ee7a c\u00e1c d\u1ea3i DNA \u0111\u01b0\u1ee3c c\u1ea3i thi\u1ec7n c\u00f3 th\u1ec3 \u0111\u1ea1t \u0111\u01b0\u1ee3c b\u1eb1ng c\u00e1ch cho ph\u00e9p gel b\u1ecb ph\u00e2n h\u1ee7y trong khay s\u1ea1ch m\u1edbi v\u00e0 ph\u1ee7 n\u01b0\u1edbc s\u1ea1ch.\u00a0\u0110\u1ec3 c\u00f3 k\u1ebft qu\u1ea3 t\u1ed1t nh\u1ea5t, h\u00e3y \u0111\u1ec3 kho\u1ea3ng 20 ph\u00fat tr\u00ean m\u00e1y l\u1eafc v\u1edbi chuy\u1ec3n \u0111\u1ed9ng l\u1eafc ch\u1eadm.\u00a0Thay n\u01b0\u1edbc trong th\u1eddi gian n\u00e0y c\u0169ng s\u1ebd c\u1ea3i thi\u1ec7n ho\u1ea1t \u0111\u1ed9ng kh\u1eed m\u00e0u.<\/p>\n
Ngo\u00e0i ra, DNA c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c nhu\u1ed9m b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng ch\u1ea5t c\u00f4 \u0111\u1eb7c xanh methylene 10x.\u00a0\u0110\u1ed5 \u0111\u1ee7 10x methylene blue \u0111\u1ec3 ph\u1ee7 gel agarose v\u00e0 \u0111\u1ec3 y\u00ean trong 5-10 ph\u00fat.\u00a0Sau khi nhu\u1ed9m xong, r\u1eeda gel agarose trong n\u01b0\u1edbc s\u1ea1ch.\u00a0\u0110\u1ed9 ph\u00e2n gi\u1ea3i c\u1ee7a c\u00e1c d\u1ea3i DNA \u0111\u01b0\u1ee3c c\u1ea3i thi\u1ec7n c\u00f3 th\u1ec3 \u0111\u1ea1t \u0111\u01b0\u1ee3c b\u1eb1ng c\u00e1ch cho ph\u00e9p gel b\u1ecb ph\u00e2n h\u1ee7y trong n\u01b0\u1edbc s\u1ea1ch.<\/p>\n
Sau khi gel c\u1ee7a b\u1ea1n \u0111\u00e3 \u0111\u01b0\u1ee3c nhu\u1ed9m v\u00e0 t\u1ea9y m\u00e0u, b\u1ea1n c\u00f3 th\u1ec3 h\u00ecnh dung c\u00e1c d\u1ea3i c\u1ee7a m\u00ecnh b\u1eb1ng c\u00e1ch \u0111\u1eb7t gel l\u00ean b\u00e0n c\u00f3 \u00e1nh s\u00e1ng ho\u1eb7c n\u1ebfu kh\u00f4ng c\u00f3 s\u1eb5n, h\u00e3y \u0111\u1eb7t gel l\u00ean m\u1ed9t b\u1ec1 m\u1eb7t m\u00e0u tr\u1eafng.<\/p>\n","protected":false},"excerpt":{"rendered":"
Thu\u1ed1c nhu\u1ed9m DNA \u0111\u1eadm \u0111\u1eb7c m\u00e0 khi \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u1edf d\u1ea1ng n\u00e0y, c\u00e1c m\u1eabu c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c nhu\u1ed9m trong v\u00f2ng 5-10 ph\u00fat<\/p>\n","protected":false},"featured_media":7527,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_cat":[21],"product_tag":[3916,3964,3962],"class_list":{"0":"post-7526","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-san-pham-khac","7":"product_tag-cleaver-scientific","8":"product_tag-problue","9":"product_tag-thuoc-nhuom-colorimetric-dna-120ml-10x","10":"yith_product_brand-cleaver-scientific","12":"first","13":"instock","14":"shipping-taxable","15":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/product\/7526"}],"collection":[{"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/comments?post=7526"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/media\/7527"}],"wp:attachment":[{"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/media?parent=7526"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/product_cat?post=7526"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/product_tag?post=7526"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}