{"id":10218,"date":"2023-11-16T09:01:57","date_gmt":"2023-11-16T09:01:57","guid":{"rendered":"https:\/\/vospan.com\/?p=10218"},"modified":"2024-03-19T02:54:49","modified_gmt":"2024-03-19T02:54:49","slug":"phan-loai-hat-nano-bang-cach-su-dung-may-phan-loai-te-bao-cytoflex-srt","status":"publish","type":"post","link":"https:\/\/vospan.com\/phan-loai-hat-nano-bang-cach-su-dung-may-phan-loai-te-bao-cytoflex-srt\/","title":{"rendered":"Ph\u00e2n lo\u1ea1i h\u1ea1t nano b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng m\u00e1y ph\u00e2n lo\u1ea1i t\u1ebf b\u00e0o CytoFLEX SRT"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_68_1 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<label class=\"ez-toc-title ez-toc-toggle\" style=\"cursor: pointer\">T&oacute;m t\u1eaft n\u1ed9i dung<\/label>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 eztoc-toggle-hide-by-default' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/vospan.com\/phan-loai-hat-nano-bang-cach-su-dung-may-phan-loai-te-bao-cytoflex-srt\/#Vat_lieu_Phuong_phap\" title=\"V\u1eadt li\u1ec7u &amp; Ph\u01b0\u01a1ng ph\u00e1p\">V\u1eadt li\u1ec7u &amp; Ph\u01b0\u01a1ng ph\u00e1p<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/vospan.com\/phan-loai-hat-nano-bang-cach-su-dung-may-phan-loai-te-bao-cytoflex-srt\/#Cai_dat_thiet_bi\" title=\"C\u00e0i \u0111\u1eb7t thi\u1ebft b\u1ecb\">C\u00e0i \u0111\u1eb7t thi\u1ebft b\u1ecb<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/vospan.com\/phan-loai-hat-nano-bang-cach-su-dung-may-phan-loai-te-bao-cytoflex-srt\/#Chuan_bi_hat_NIST_hat_huynh_quang_VLP_vi_khuan\" title=\"Chu\u1ea9n b\u1ecb h\u1ea1t NIST, h\u1ea1t hu\u1ef3nh quang, VLP, vi khu\u1ea9n\">Chu\u1ea9n b\u1ecb h\u1ea1t NIST, h\u1ea1t hu\u1ef3nh quang, VLP, vi khu\u1ea9n<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/vospan.com\/phan-loai-hat-nano-bang-cach-su-dung-may-phan-loai-te-bao-cytoflex-srt\/#Phan_tich_sau_sap_xep\" title=\"Ph\u00e2n t\u00edch sau s\u1eafp x\u1ebfp\">Ph\u00e2n t\u00edch sau s\u1eafp x\u1ebfp<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/vospan.com\/phan-loai-hat-nano-bang-cach-su-dung-may-phan-loai-te-bao-cytoflex-srt\/#Ket_qua\" title=\"K\u1ebft qu\u1ea3\">K\u1ebft qu\u1ea3<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/vospan.com\/phan-loai-hat-nano-bang-cach-su-dung-may-phan-loai-te-bao-cytoflex-srt\/#Phan_loai_vi_khuan_don_%3E250%C2%B5m\" title=\"Ph\u00e2n lo\u1ea1i vi khu\u1ea9n \u0111\u01a1n (&gt;250\u00b5m)\">Ph\u00e2n lo\u1ea1i vi khu\u1ea9n \u0111\u01a1n (&gt;250\u00b5m)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/vospan.com\/phan-loai-hat-nano-bang-cach-su-dung-may-phan-loai-te-bao-cytoflex-srt\/#Phan_loai_hat_nano_YG_100nm\" title=\"Ph\u00e2n lo\u1ea1i h\u1ea1t nano YG 100nm\">Ph\u00e2n lo\u1ea1i h\u1ea1t nano YG 100nm<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/vospan.com\/phan-loai-hat-nano-bang-cach-su-dung-may-phan-loai-te-bao-cytoflex-srt\/#Phan_loai_eGFP_va_tdTomato_co_nhan_VLP\" title=\"Ph\u00e2n lo\u1ea1i eGFP v\u00e0 tdTomato c\u00f3 nh\u00e3n VLP\">Ph\u00e2n lo\u1ea1i eGFP v\u00e0 tdTomato c\u00f3 nh\u00e3n VLP<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/vospan.com\/phan-loai-hat-nano-bang-cach-su-dung-may-phan-loai-te-bao-cytoflex-srt\/#Phan_ket_luan\" title=\"Ph\u1ea7n k\u1ebft lu\u1eadn\">Ph\u1ea7n k\u1ebft lu\u1eadn<\/a><\/li><\/ul><\/nav><\/div>\n<p>Ph\u01b0\u01a1ng ph\u00e1p <a href=\"https:\/\/vospan.com\/product-category\/cong-nghe-sinh-hoc\/sinh-hoc-phan-tu\/may-dem-te-bao-dong-chay\/\">\u0111\u1ebfm t\u1ebf b\u00e0o d\u00f2ng ch\u1ea3y<\/a> \u0111\u1ec3\u00a0ph\u00e2n lo\u1ea1i h\u1ea1t nano l\u00e0 m\u1ed9t trong m\u1ed9t s\u1ed1 k\u1ef9 thu\u1eadt \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 m\u00f4 t\u1ea3 \u0111\u1eb7c t\u00ednh c\u1ee7a c\u00e1c h\u1ea1t si\u00eau nh\u1ecf. C\u00e1c h\u1ea1t sinh h\u1ecdc th\u01b0\u1eddng \u0111\u01b0\u1ee3c kh\u00e1m ph\u00e1 trong ph\u1ea1m vi k\u00edch th\u01b0\u1edbc n\u00e0y bao g\u1ed3m vi khu\u1ea9n, vi r\u00fat, t\u00fai ngo\u1ea1i b\u00e0o v\u00e0 exosome. C\u00f4ng c\u1ee5 ph\u00e2n t\u00edch n\u00e0y \u0111\u00e3 tr\u1edf n\u00ean ph\u1ed5 bi\u1ebfn h\u01a1n nh\u1edd kh\u1ea3 n\u0103ng th\u00f4ng l\u01b0\u1ee3ng cao v\u00e0 \u0111a th\u00f4ng s\u1ed1. Tr\u01b0\u1edbc \u0111\u00e2y ng\u01b0\u1eddi ta \u0111\u00e3 ch\u1ee9ng minh r\u1eb1ng M\u00e1y \u0111\u1ebfm t\u1ebf b\u00e0o d\u00f2ng ch\u1ea3y\u00a0<a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-may-dem-te-bao-dong-chay-trong-nghien-cuu-2\/\">CytoFLEX<\/a> c\u1ee7a <a href=\"https:\/\/vospan.com\/product-brands\/beckman-coulter\/\">Beckman Coulter<\/a> c\u00f3 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n c\u00e1c h\u1ea1t nano polystyrene 80 nm b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng tham s\u1ed1 Violet Side Scatter. Do c\u00e1c th\u00e0nh ph\u1ea7n thi\u1ebft k\u1ebf quang h\u1ecdc, gh\u00e9p k\u00eanh ph\u00e2n chia b\u01b0\u1edbc s\u00f3ng, \u0111i\u1ed1t tuy\u1ebft l\u1edf v\u00e0 laser diode, <a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-may-dem-te-bao-dong-chay-trong-nghien-cuu-2\/\">CytoFLEX<\/a> \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ee9ng minh l\u00e0 c\u00f3 m\u1ee9c \u0111\u1ed9 t\u00e1n x\u1ea1 \u00e1nh s\u00e1ng v\u00e0 \u0111\u1ed9 nh\u1ea1y hu\u1ef3nh quang \u0111\u01b0\u1ee3c n\u00e2ng cao.V\u1edbi nh\u1eefng ti\u1ebfn b\u1ed9 k\u1ef9 thu\u1eadt quang h\u1ecdc v\u00e0 s\u1ef1 gia t\u0103ng s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y \u0111\u1ec3 ph\u00e2n t\u00edch EV, \u0111\u00e3 c\u00f3 nh\u1eefng n\u1ed7 l\u1ef1c nh\u1eb1m chu\u1ea9n h\u00f3a c\u00e1c ph\u01b0\u01a1ng ph\u00e1p t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y c\u00f3 k\u00edch th\u01b0\u1edbc nano. S\u1ef1 ch\u1eb7t ch\u1ebd trong vi\u1ec7c \u00e1p d\u1ee5ng s\u1ee9c m\u1ea1nh c\u1ee7a \u0111\u1eb7c t\u00ednh h\u1ea1t \u0111\u01a1n, th\u00f4ng l\u01b0\u1ee3ng cao, g\u00f3p ph\u1ea7n \u0111\u00e1ng k\u1ec3 v\u00e0o nh\u1eefng ti\u1ebfn b\u1ed9 trong mi\u1ec5n d\u1ecbch t\u1ebf b\u00e0o, sau \u0111\u00f3 c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng cho nghi\u00ean c\u1ee9u EV.M\u1eb7c d\u00f9 nh\u1eefng n\u1ed7 l\u1ef1c n\u00e0y \u0111\u00e3 \u0111\u1ea1t \u0111\u01b0\u1ee3c ti\u1ebfn b\u1ed9 trong ph\u00e2n t\u00edch t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y \u1edf quy m\u00f4 nano, nh\u01b0ng vi\u1ec7c ph\u00e2n lo\u1ea1i h\u1ea1t nano v\u1eabn l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt ph\u1ee9c t\u1ea1p kh\u00f4ng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn. Tr\u01b0\u1edbc \u0111\u00e2y, ng\u01b0\u1eddi ta \u0111\u00e3 ch\u1ee9ng minh tr\u00ean MoFlo Astrios r\u1eb1ng c\u00e1c t\u00fai c\u00f3 k\u00edch th\u01b0\u1edbc nano c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eafp x\u1ebfp v\u1edbi hi\u1ec7u su\u1ea5t t\u1eeb \u200b\u200b25 \u0111\u1ebfn 45%. Tuy nhi\u00ean, thi\u1ebft k\u1ebf MoFlo Astrios l\u00e0 m\u1ed9t \u0111\u1ecbnh d\u1ea1ng l\u1edbn v\u1edbi c\u00e1c y\u00eau c\u1ea7u kh\u1ed1i l\u01b0\u1ee3ng c\u00f4ng vi\u1ec7c ph\u1ee9c t\u1ea1p \u0111\u1ec3 thi\u1ebft l\u1eadp v\u00e0 ch\u1ea1y lo\u1ea1i c\u00f3 k\u00edch th\u01b0\u1edbc nano.Do thi\u1ebft k\u1ebf b\u1ed5 sung c\u1ee7a m\u00e1y ph\u00e2n lo\u1ea1i t\u1ebf b\u00e0o <a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-srt-may-phan-loai-te-bao-de-ban\/\">CytoFLEX SRT<\/a> v\u00e0 m\u00e1y ph\u00e2n t\u00edch t\u1ebf b\u00e0o CytoFLEX S (c\u1ea3 hai \u0111\u1ec1u s\u1eed d\u1ee5ng m\u00e1y d\u00f2 APD, WDM v\u00e0 ph\u00e1t hi\u1ec7n VSSC 1), c\u00e1c \u0111\u1eb7c t\u00ednh hi\u1ec7u su\u1ea5t d\u1ef1 ki\u1ebfn \u200b\u200bs\u1ebd t\u01b0\u01a1ng t\u1ef1 nhau. V\u00ec v\u1eady, ng\u01b0\u1eddi ta ngh\u0129 r\u1eb1ng vi\u1ec7c ph\u00e2n lo\u1ea1i h\u1ea1t nano l\u00e0 kh\u1ea3 thi. Ngo\u00e0i ra, thi\u1ebft l\u1eadp s\u1eafp x\u1ebfp t\u1ef1 \u0111\u1ed9ng v\u00e0 thi\u1ebft l\u1eadp c\u00f4ng c\u1ee5 t\u1ed1i thi\u1ec3u c\u1ea7n thi\u1ebft \u0111\u1ec3 th\u1ef1c hi\u1ec7n ph\u01b0\u01a1ng ph\u00e1p t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y \u1edf quy m\u00f4 nano c\u00f3 th\u1ec3 cho ph\u00e9p s\u1eed d\u1ee5ng m\u00e1y ph\u00e2n lo\u1ea1i t\u1ebf b\u00e0o <a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-srt-may-phan-loai-te-bao-de-ban\/\">CytoFLEX SRT<\/a> l\u00e0m c\u00f4ng c\u1ee5 cho c\u00e1c th\u00ed nghi\u1ec7m \u0111\u01b0\u1ee3c ti\u00eau chu\u1ea9n h\u00f3a v\u00e0 t\u00e1i s\u1ea3n xu\u1ea5t.<\/p>\n<p>Trong c\u00e1c th\u00ed nghi\u1ec7m sau \u0111\u00e2y, <a href=\"https:\/\/vospan.com\/product-brands\/beckman-coulter\/\">Beckman Coulter<\/a> m\u00f4 t\u1ea3 kh\u1ea3 n\u0103ng c\u1ee7a m\u00e1y ph\u00e2n lo\u1ea1i t\u1ebf b\u00e0o<a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-srt-may-phan-loai-te-bao-de-ban\/\"> CytoFLEX SRT<\/a> \u0111\u1ec3 ph\u00e2n lo\u1ea1i c\u00e1c m\u1eabu c\u00f3 k\u00edch th\u01b0\u1edbc nano. K\u1ebft qu\u1ea3 cho th\u1ea5y hi\u1ec7u qu\u1ea3 cao h\u01a1n trong vi\u1ec7c ph\u00e2n lo\u1ea1i h\u1ea1t nano so v\u1edbi c\u00e1c b\u00e1o c\u00e1o hi\u1ec7n c\u00f3 v\u00e0 m\u00e1y ph\u00e2n lo\u1ea1i t\u1ebf b\u00e0o <a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-srt-may-phan-loai-te-bao-de-ban\/\">CytoFLEX SRT<\/a> c\u00f3 th\u1ec3 ph\u00e2n lo\u1ea1i \u0111\u01b0\u1ee3c m\u1ed9t s\u1ed1 lo\u1ea1i h\u1ea1t nano.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Vat_lieu_Phuong_phap\"><\/span><strong>V\u1eadt li\u1ec7u &amp; Ph\u01b0\u01a1ng ph\u00e1p<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<h3><span class=\"ez-toc-section\" id=\"Cai_dat_thiet_bi\"><\/span><strong>C\u00e0i \u0111\u1eb7t thi\u1ebft b\u1ecb<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>Kh\u1edfi \u0111\u1ed9ng v\u00e0 QC h\u00e0ng ng\u00e0y \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n theo h\u01b0\u1edbng d\u1eabn trong H\u01b0\u1edbng d\u1eabn s\u1eed d\u1ee5ng m\u00e1y ph\u00e2n lo\u1ea1i t\u1ebf b\u00e0o <a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-srt-may-phan-loai-te-bao-de-ban\/\">CytoFLEX SRT<\/a>.\u00a0T\u00f3m l\u1ea1i, sau khi b\u1eadt thi\u1ebft b\u1ecb, ch\u01b0\u01a1ng tr\u00ecnh kh\u1edfi \u0111\u1ed9ng h\u1ec7 th\u1ed1ng s\u1ebd \u0111\u01b0\u1ee3c ch\u1ea1y v\u00e0 \u0111\u1ea7u phun \u0111\u01b0\u1ee3c l\u1eafp v\u00e0o. Sau khi kh\u1edfi \u0111\u1ed9ng xong, c\u1ea7u hu\u1ef3nh quang CytoFLEX Daily QC \u0111\u01b0\u1ee3c \u0111\u1eb7t l\u00ean tr\u1ea1m m\u1eabu v\u00e0 thi\u1ebft b\u1ecb \u0111\u01b0\u1ee3c h\u01b0\u1edbng d\u1eabn ch\u1ea1y QC. Sau khi v\u01b0\u1ee3t qua QC, thi\u1ebft b\u1ecb \u0111\u01b0\u1ee3c c\u1ea5u h\u00ecnh \u0111\u1ec3 ph\u00e1t hi\u1ec7n Ph\u00e2n t\u00e1n b\u00ean (SSC) b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng tia laser Violet. Do c\u00e1c b\u1ed9 ph\u1eadn quang h\u1ecdc c\u1ee7a m\u00e1y \u0111o t\u1ebf b\u00e0o d\u00f2ng ch\u1ea3y CytoFLEX S v\u00e0 m\u00e1y ph\u00e2n lo\u1ea1i t\u1ebf b\u00e0o <a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-srt-may-phan-loai-te-bao-de-ban\/\">CytoFLEX SRT<\/a>\u00a0kh\u00f4ng c\u1ea7n tr\u1ea3 ph\u00ed n\u00ean quy tr\u00ecnh n\u00e0y c\u00f3 gi\u00e1 tr\u1ecb tr\u00ean c\u1ea3 hai thi\u1ebft b\u1ecb. \u0110i\u1ec1u n\u00e0y \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n d\u1ec5 d\u00e0ng nh\u01b0 ho\u00e1n \u0111\u1ed5i v\u1ecb tr\u00ed WDM c\u1ee7a b\u1ed9 l\u1ecdc 405\/10 v\u00e0 b\u1ed9 l\u1ecdc 450, do \u0111\u00f3 lo\u1ea1i b\u1ecf k\u00eanh Pacific Blue v\u00e0 t\u1ea1o k\u00eanh Violet Side Scatter (VSSC).\u00a0Vi\u1ec7c ho\u00e1n \u0111\u1ed5i l\u1ea1i c\u00e1c b\u1ed9 l\u1ecdc s\u1ebd cho ph\u00e9p \u0111\u1ea3o ng\u01b0\u1ee3c c\u1ea5u h\u00ecnh ban \u0111\u1ea7u.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Chuan_bi_hat_NIST_hat_huynh_quang_VLP_vi_khuan\"><\/span><strong>Chu\u1ea9n b\u1ecb h\u1ea1t NIST, h\u1ea1t hu\u1ef3nh quang, VLP, vi khu\u1ea9n<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>T\u1ea5t c\u1ea3 c\u00e1c h\u1ea1t NIST (Polysciences, Inc., Warrington, PA) \u0111\u00e3 \u0111\u01b0\u1ee3c chu\u1ea9n b\u1ecb nh\u01b0 m\u00f4 t\u1ea3 trong FCMPASS &#8211; Thu th\u1eadp v\u00e0 ch\u1ecdn l\u1ecdc v\u1eadt li\u1ec7u tham chi\u1ebfu t\u00e1n x\u1ea1 \u00e1nh s\u00e1ng V.2.\u00a0T\u00f3m l\u1ea1i, m\u1ed7i chai g\u1ed1c \u0111\u01b0\u1ee3c tr\u1ed9n v\u00e0 sau \u0111\u00f3 pha lo\u00e3ng \u0111\u1ebfn n\u1ed3ng \u0111\u1ed9 1&#215;107 trong\u00a0500 \u00b5mL DPBS.\u00a0Sau \u0111\u00f3, c\u00e1c h\u1ea1t \u0111\u01b0\u1ee3c thu th\u1eadp t\u1ed5ng c\u1ed9ng 10.000 s\u1ef1 ki\u1ec7n trong c\u1ed5ng \u0111\u01b0\u1ee3c ch\u1ec9 \u0111\u1ecbnh b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng c\u1ea5u h\u00ecnh VSSC tr\u00ean m\u00e1y ph\u00e2n lo\u1ea1i t\u1ebf b\u00e0o <a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-srt-may-phan-loai-te-bao-de-ban\/\">CytoFLEX SRT<\/a>.<\/p>\n<p>VLP \u0111\u01b0\u1ee3c g\u1eafn th\u1ebb eGFP v\u00e0 tdTomato \u0111\u00e3 \u0111\u01b0\u1ee3c chu\u1ea9n b\u1ecb v\u00e0 cung c\u1ea5p b\u1edfi Ti\u1ebfn s\u0129 Rahm Gummuluru.\u00a0T\u00f3m l\u1ea1i, bi\u1ec3u hi\u1ec7n plasmit HIV-1 pGag-eGFP v\u00e0\/ho\u1eb7c pGag-tdTomato bi\u1ec3u hi\u1ec7n protein hu\u1ef3nh quang m\u00e0u xanh l\u00e1 c\u00e2y \u0111\u01b0\u1ee3c t\u0103ng c\u01b0\u1eddng Gag (eGFP) v\u00e0\/ho\u1eb7c protein t\u1ed5ng h\u1ee3p protein hu\u1ef3nh quang tdTomato, \u0111\u00e3 \u0111\u01b0\u1ee3c m\u00f4 t\u1ea3 tr\u01b0\u1edbc \u0111\u00e2y (Cat # ARP-11468 NIH Ch\u01b0\u01a1ng tr\u00ecnh Thu\u1ed1c th\u1eed HIV, Ph\u00f2ng AIDS, NIAID, NIH, do Marilyn D. Resh v\u00e0 George Pavlakis \u0111\u00f3ng g\u00f3p).\u00a0HIV Gag-eGFP\/tdTomato, c\u00e1c h\u1ea1t gi\u1ed1ng vi-r\u00fat (VLP) \u0111\u01b0\u1ee3c t\u1ea1o ra th\u00f4ng qua qu\u00e1 tr\u00ecnh chuy\u1ec3n h\u00f3a qua trung gian canxi photphat c\u1ee7a c\u00e1c t\u1ebf b\u00e0o HEK293T.\u00a0Sau khi thu ho\u1ea1ch, ch\u1ea5t n\u1ed5i ph\u00eda tr\u00ean ch\u1ee9a VLP \u0111\u01b0\u1ee3c l\u00e0m s\u1ea1ch kh\u1ecfi c\u00e1c m\u1ea3nh v\u1ee5n t\u1ebf b\u00e0o, \u0111i qua b\u1ed9 l\u1ecdc 0,45-\u00b5m M v\u00e0 \u0111\u01b0\u1ee3c t\u1ea1o th\u00e0nh vi\u00ean qua \u0111\u1ec7m sucrose 20%.\u00a0N\u1ed9i dung Gag p24 c\u1ee7a VLP \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh b\u1eb1ng ELISA.<\/p>\n<p>C\u00e1c ch\u1ee7ng<em> Bacillus subtilis<\/em>\u00a0\u0111\u01b0\u1ee3c t\u1ea1o ra b\u1eb1ng c\u00e1ch bi\u1ebfn n\u1ea1p th\u00e0nh\u00a0<em>B. subtilis 168 trpC2<\/em>\u00a0(PB2) v\u00e0 c\u00e1c d\u1eabn xu\u1ea5t c\u1ee7a n\u00f3 tr\u1eeb khi c\u00f3 ghi ch\u00fa kh\u00e1c.\u00a0C\u00e1c ch\u1ee7ng B. subtilis \u0111\u01b0\u1ee3c bi\u1ebfn n\u1ea1p v\u1edbi 5\u201310 \u00b5mL DNA plasmid ho\u1eb7c 1\u20132 \u00b5L DNA b\u1ed9 gen b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p hai b\u01b0\u1edbc.\u00a0DNA b\u1ed9 gen \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ebf b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng B\u1ed9 l\u1ecdc DNA b\u1ed9 gen Wizard (Promega) theo h\u01b0\u1edbng d\u1eabn c\u1ee7a nh\u00e0 s\u1ea3n xu\u1ea5t.\u00a0C\u00e1c n\u1ec1n v\u0103n h\u00f3a \u0111\u01b0\u1ee3c tr\u1ed3ng \u1edf LB (Lennox).<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Phan_tich_sau_sap_xep\"><\/span><strong>Ph\u00e2n t\u00edch sau s\u1eafp x\u1ebfp<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>T\u1ea5t c\u1ea3 h\u00ecnh \u1ea3nh \u0111\u01b0\u1ee3c ch\u1ee5p b\u1eb1ng k\u00ednh hi\u1ec3n vi Olympus BX62 (T\u1eadp \u0111o\u00e0n Olympus, Tokyo, Nh\u1eadt B\u1ea3n). H\u00ecnh \u1ea3nh \u0111\u01b0\u1ee3c ch\u1ee5p b\u1eb1ng m\u00e1y \u1ea3nh Andor Sona \u1edf v\u1eadt k\u00ednh 40,0x v\u1edbi d\u1ea7u ng\u00e2m.\u00a0C\u1ea3 k\u00eanh \u0111\u01a1n FITC v\u00e0 k\u00eanh \u0111\u01a1n Texas Red \u0111\u1ec1u \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u1edbi \u0111\u1ed9 ph\u01a1i s\u00e1ng \u0111\u01b0\u1ee3c \u0111\u1eb7t th\u00e0nh 100 ms.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Ket_qua\"><\/span><strong>K\u1ebft qu\u1ea3<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Trong ph\u1ea7n n\u00e0y s\u1ebd m\u00f4 t\u1ea3 c\u00e1ch s\u1eafp x\u1ebfp c\u00e1c th\u1ef1c th\u1ec3 kh\u00e1c nhau c\u00f3 k\u00edch th\u01b0\u1edbc kh\u00e1c nhau, v\u1edbi k\u00edch th\u01b0\u1edbc gi\u1ea3m d\u1ea7n v\u00e0 \u0111\u1ed9 ph\u1ee9c t\u1ea1p t\u0103ng d\u1ea7n. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p chi ti\u1ebft \u0111\u01b0\u1ee3c m\u00f4 t\u1ea3 trong ph\u1ea7n tr\u01b0\u1edbc.\u00a0S\u1ef1 \u0111a d\u1ea1ng c\u1ee7a c\u00e1c th\u00ed nghi\u1ec7m s\u1eafp x\u1ebfp \u0111\u01b0\u1ee3c m\u00f4 t\u1ea3 \u1edf \u0111\u00e2y nh\u1eb1m m\u1ee5c \u0111\u00edch ch\u1ee9ng minh t\u00ednh linh ho\u1ea1t c\u1ee7a m\u00e1y ph\u00e2n lo\u1ea1i t\u1ebf b\u00e0o <a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-srt-may-phan-loai-te-bao-de-ban\/\">CytoFLEX SRT<\/a>.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Phan_loai_vi_khuan_don_%3E250%C2%B5m\"><\/span><strong>Ph\u00e2n lo\u1ea1i vi khu\u1ea9n \u0111\u01a1n (&gt;250\u00b5m)<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>M\u1ed9t m\u1eabu\u00a0<em>Bacillus subtilis<\/em> \u0111\u01b0\u1ee3c k\u00edch ho\u1ea1t c\u00f3 nh\u00e3n YFP\u00a0\u0111\u01b0\u1ee3c ph\u00e2n t\u00edch b\u1eb1ng k\u00eanh FITC (525\/40).\u00a0Vi khu\u1ea9n d\u01b0\u01a1ng t\u00ednh \u0111\u01b0\u1ee3c ki\u1ec3m so\u00e1t v\u00e0 sau \u0111\u00f3 m\u1ed9t vi khu\u1ea9n duy nh\u1ea5t \u0111\u01b0\u1ee3c s\u1eafp x\u1ebfp tr\u00ean m\u1ed9t phi\u1ebfn k\u00ednh v\u00e0 \u0111\u01b0\u1ee3c x\u00e1c minh b\u1eb1ng k\u00ednh hi\u1ec3n vi hu\u1ef3nh quang.\u00a0Th\u1eed nghi\u1ec7m \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n \u0111\u1ec3 x\u00e1c minh kh\u1ea3 n\u0103ng c\u1ee7a m\u00e1y trong vi\u1ec7c ph\u00e2n lo\u1ea1i sinh v\u1eadt t\u01b0\u01a1ng \u0111\u1ed1i nh\u1ecf (d\u00e0i 2-6 \u00b5m v\u00e0 \u0111\u01b0\u1eddng k\u00ednh 0,25\u20131,0 \u00b5m) c\u00f3 ch\u1ec9 s\u1ed1 kh\u00fac x\u1ea1 t\u01b0\u01a1ng t\u1ef1 nh\u01b0 EV l\u00e0 1,38 4.<\/p>\n<figure style=\"width: 781px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.mybeckman.vn\/resources\/reading-material\/application-notes\/nanoscale-sorting-cytoflex-srt##\" data-featherlight=\"https:\/\/media.beckman.com\/-\/media\/flow-cytometry\/products\/instruments\/srt\/application-note\/nanoscale-sorting-cytoflex-srt-gating-strategy.png\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"center\" src=\"https:\/\/media.beckman.com\/-\/media\/flow-cytometry\/products\/instruments\/srt\/application-note\/nanoscale-sorting-cytoflex-srt-gating-strategy.png\" alt=\"Ph\u00e2n lo\u1ea1i CytoFLEX SRT B. subtilis\" width=\"781\" height=\"290\" \/><\/a><figcaption class=\"wp-caption-text\">H\u00ecnh 1. Ph\u00e2n\u00a0lo\u1ea1i th\u1eed nghi\u1ec7m B. subtilis<\/figcaption><\/figure>\n<p>Vi khu\u1ea9n nu\u00f4i c\u1ea5y \u0111\u01b0\u1ee3c s\u1eafp x\u1ebfp theo c\u00e1c v\u00f9ng ph\u00e2n lo\u1ea1i \u0111\u1ec3 lo\u1ea1i b\u1ecf m\u1ea3nh v\u1ee5n v\u00e0 vi khu\u1ea9n k\u00e9p, v\u00e0 cu\u1ed1i c\u00f9ng l\u00e0 vi khu\u1ea9n d\u01b0\u01a1ng t\u00ednh v\u1edbi YFP (Ng\u01b0\u1ee1ng VSSC 1650\/Gain 300, FITC-750, SSC-100).\u00a0Sau khi l\u1eafng \u0111\u1ecdng tr\u00ean phi\u1ebfn k\u00ednh v\u00e0 hi\u1ec3n th\u1ecb b\u1eb1ng k\u00ednh hi\u1ec3n vi Olympus BX62.\u00a0H\u00ecnh \u1ea3nh \u0111\u01b0\u1ee3c ch\u1ee5p b\u1eb1ng m\u00e1y \u1ea3nh Andor Sona \u1edf v\u1eadt k\u00ednh 40x v\u1edbi d\u1ea7u ng\u00e2m.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Phan_loai_hat_nano_YG_100nm\"><\/span><strong>Ph\u00e2n lo\u1ea1i h\u1ea1t nano YG 100nm<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>Ti\u1ebfp theo, c\u00e1c h\u1ea1t YG Polystyrene 100 nm \u0111\u01b0\u1ee3c tr\u1ed9n v\u1edbi c\u00e1c h\u1ea1t kh\u00f4ng hu\u1ef3nh quang 150 nm.\u00a0C\u00e1c h\u1ea1t 100nm \u0111\u01b0\u1ee3c s\u1eafp x\u1ebfp n\u0103m h\u1ea1t tr\u00ean m\u1ed9t ti\u00eau b\u1ea3n v\u00e0 m\u1ed9t h\u1ea1t duy nh\u1ea5t tr\u00ean m\u1ed9t ti\u00eau b\u1ea3n. Ng\u01b0\u1ee1ng \u0111\u01b0\u1ee3c \u0111\u1eb7t \u0111\u1ee7 th\u1ea5p (Ng\u01b0\u1ee1ng VSSC 3000\/T\u0103ng 300, FITC-400, SSC-500) \u0111\u1ec3 tr\u1ef1c quan h\u00f3a hai qu\u1ea7n th\u1ec3 h\u1ea1t v\u00e0 lo\u1ea1i b\u1ecf c\u00e1c s\u1ef1 ki\u1ec7n nhi\u1ec5u \u0111i\u1ec7n t\u1eed v\u00e0 ch\u1ea5t l\u1ecfng.\u00a0Lo\u1ea1i th\u1eed nghi\u1ec7m n\u00e0y \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh xem thi\u1ebft b\u1ecb c\u00f3 kh\u1ea3 n\u0103ng ph\u00e2n lo\u1ea1i c\u00e1c h\u1ea1t nano b\u1eb1ng ti\u00eau chu\u1ea9n phi sinh h\u1ecdc hay kh\u00f4ng.<\/p>\n<figure style=\"width: 526px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.mybeckman.vn\/resources\/reading-material\/application-notes\/nanoscale-sorting-cytoflex-srt##\" data-featherlight=\"https:\/\/media.beckman.com\/-\/media\/flow-cytometry\/products\/instruments\/srt\/application-note\/nanoscale-sorting-cytoflex-srt-yg-bead-sort.png\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"center\" src=\"https:\/\/media.beckman.com\/-\/media\/flow-cytometry\/products\/instruments\/srt\/application-note\/nanoscale-sorting-cytoflex-srt-yg-bead-sort.png\" alt=\"CytoFLEX SRT Ph\u00e2n lo\u1ea1i h\u1ea1t YG 100 nm\" width=\"526\" height=\"495\" \/><\/a><figcaption class=\"wp-caption-text\">H\u00ecnh 2. Ph\u00e2n lo\u1ea1i h\u1ea1t YG 100 nm<\/figcaption><\/figure>\n<p>C\u00e1c h\u1ea1t 100 nm \u0111\u01b0\u1ee3c ph\u00e2n t\u00e1ch b\u1eb1ng v\u00f9ng 100 nm tr\u00ean tham s\u1ed1 VSSC <strong>(tr\u00ean c\u00f9ng)<\/strong>.\u00a0Sau khi l\u1eafng \u0111\u1ecdng tr\u00ean m\u1ed9t lam k\u00ednh, c\u00e1c h\u1ea1t \u0111\u01b0\u1ee3c ch\u1ee5p \u1ea3nh \u1edf \u0111\u1ed9 ph\u00f3ng \u0111\u1ea1i 40 l\u1ea7n <strong>(ph\u00eda d\u01b0\u1edbi)<\/strong>\u00a0.\u00a0Vi\u1ec7c s\u1eafp x\u1ebfp cho c\u1ea3 5 h\u1ea1t m\u1ed7i v\u1ecb tr\u00ed\u00a0<strong>(tr\u00e1i)<\/strong>\u00a0v\u00e0 1 h\u1ea1t m\u1ed7i v\u1ecb tr\u00ed\u00a0<strong>(ph\u1ea3i)<\/strong>\u00a0\u0111\u00e3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n.\u00a0M\u0169i t\u00ean tr\u00ean m\u1ed7i h\u00ecnh \u1ea3nh ch\u1ec9 v\u1ecb tr\u00ed c\u1ee7a h\u1ea1t 100nm.\u00a0L\u01b0u \u00fd m\u1ee9c n\u1ec1n cao do c\u00e1c tinh th\u1ec3 mu\u1ed1i h\u00ecnh th\u00e0nh khi v\u1ecf kh\u00f4.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Phan_loai_eGFP_va_tdTomato_co_nhan_VLP\"><\/span><strong>Ph\u00e2n lo\u1ea1i eGFP v\u00e0 tdTomato c\u00f3 nh\u00e3n VLP<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>C\u00e1c h\u1ea1t VLP \u0111\u01b0\u1ee3c d\u00e1n nh\u00e3n eGFP v\u00e0 tdTomato (100-120 nm, RI 1.369 <sup>5<\/sup>\u00a0) \u0111\u00f3ng vai tr\u00f2 l\u00e0 c\u00e1c bi\u1ec7n ph\u00e1p ki\u1ec3m so\u00e1t sinh h\u1ecdc ti\u1ebfp theo.\u00a0\u0110\u00e2y l\u00e0 nh\u1eefng h\u1ea1t nano c\u00f3 \u0111\u1eb7c t\u00ednh t\u1ed1t v\u1edbi t\u00edn hi\u1ec7u s\u00e1ng.\u00a0C\u00e1c m\u1eabu \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch tr\u00ean b\u1ed9 k\u00edch ho\u1ea1t VSSC tr\u01b0\u1edbc khi chuy\u1ec3n sang b\u1ed9 k\u00edch ho\u1ea1t hu\u1ef3nh quang t\u01b0\u01a1ng \u1ee9ng t\u1ed1i \u01b0u cho t\u1eebng lo\u1ea1i protein hu\u1ef3nh quang.\u00a0Ng\u01b0\u1ee1ng VSSC ban \u0111\u1ea7u \u0111\u01b0\u1ee3c \u0111\u1eb7t b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng c\u00e1c h\u1ea1t NIST nh\u01b0 \u0111\u01b0\u1ee3c m\u00f4 t\u1ea3 trong\u00a0giao th\u1ee9c FCM PASS.\u00a0T\u1ed1i thi\u1ec3u 100.000 s\u1ef1 ki\u1ec7n \u0111\u00e3 \u0111\u01b0\u1ee3c thu th\u1eadp.\u00a0Sau \u0111\u00f3, c\u00e1c \u1ed1ng thu th\u1eadp \u0111\u01b0\u1ee3c c\u00f4 \u0111\u1eb7c b\u1eb1ng b\u1ed9 l\u1ecdc Amicon v\u00e0 ph\u00e2n t\u00edch l\u1ea1i tr\u00ean thi\u1ebft b\u1ecb.\u00a0K\u1ebft qu\u1ea3 \u0111\u01b0\u1ee3c th\u1ec3 hi\u1ec7n trong h\u00ecnh 3 v\u00e0 4.<\/p>\n<figure style=\"width: 526px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.mybeckman.vn\/resources\/reading-material\/application-notes\/nanoscale-sorting-cytoflex-srt##\" data-featherlight=\"https:\/\/media.beckman.com\/-\/media\/flow-cytometry\/products\/instruments\/srt\/application-note\/nanoscale-sorting-cytoflex-srt-gating-strategy-2.png\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"center\" src=\"https:\/\/media.beckman.com\/-\/media\/flow-cytometry\/products\/instruments\/srt\/application-note\/nanoscale-sorting-cytoflex-srt-gating-strategy-2.png\" alt=\"CytoFLEX SRT S\u1eafp x\u1ebfp GFP+ v\u00e0 GFP-\" width=\"526\" height=\"317\" \/><\/a><figcaption class=\"wp-caption-text\">H\u00ecnh 3. Ph\u00e2n lo\u1ea1i h\u1ea1t gi\u1ed1ng virus GFP+ v\u00e0 GFP-<\/figcaption><\/figure>\n<p>\u1ed0ng thu th\u1eadp d\u01b0\u01a1ng t\u00ednh &lt;strong.(bottom&gt; hi\u1ec3n th\u1ecb &gt;96% c\u00e1c s\u1ef1 ki\u1ec7n trong c\u1ed5ng d\u01b0\u01a1ng t\u00ednh \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh tr\u01b0\u1edbc \u0111\u00f3(b\u1ea3ng gi\u1eefa tr\u00ean c\u00f9ng).\u00a0\u1ed0ng thu \u00e2m\u00a0<strong>(b\u1ea3ng d\u01b0\u1edbi c\u00f9ng b\u00ean tr\u00e1i)<\/strong>\u00a0hi\u1ec3n th\u1ecb l &lt;1% \u1edf c\u1ed5ng d\u01b0\u01a1ng.\u00a0&lt;\/strong.(bottom&gt;<\/p>\n<p>V\u00ec m\u1ee5c ti\u00eau c\u1ee7a c\u00e1c th\u00ed nghi\u1ec7m n\u00e0y l\u00e0 gh\u00e9p c\u00e1c t\u00edn hi\u1ec7u ph\u00e2n t\u1eed cho c\u00e1c miRNA kh\u00e1c nhau n\u00ean c\u00e1c m\u1eabu eGFP v\u00e0 tdTomato VLP \u0111\u00e3 \u0111\u01b0\u1ee3c k\u1ebft h\u1ee3p \u0111\u1ec3 ki\u1ec3m tra xem li\u1ec7u c\u00e1c qu\u1ea7n th\u1ec3 hu\u1ef3nh quang kh\u00e1c nhau c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c ph\u00e2n t\u00e1ch tr\u00ean SRT v\u1edbi \u0111\u1ed9 tinh khi\u1ebft cao hay kh\u00f4ng.\u00a0Sau khi ph\u00e2n lo\u1ea1i th\u00e0nh c\u00e1c \u1ed1ng thu th\u1eadp c\u1ed5ng d\u01b0\u01a1ng t\u00ednh FITC v\u00e0 PE \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch \u0111\u1ec3 ki\u1ec3m tra \u0111\u1ed9 tinh khi\u1ebft.\u00a0Qu\u1ea7n th\u1ec3 d\u01b0\u01a1ng t\u00ednh v\u1edbi tdTomato cho th\u1ea5y \u0111\u1ed9 tinh khi\u1ebft 97% v\u00e0 qu\u1ea7n th\u1ec3 d\u01b0\u01a1ng t\u00ednh v\u1edbi GFP cho th\u1ea5y \u0111\u1ed9 tinh khi\u1ebft 98%.\u00a0K\u1ebft qu\u1ea3 \u0111\u01b0\u1ee3c th\u1ec3 hi\u1ec7n trong h\u00ecnh 4.<\/p>\n<figure style=\"width: 526px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.mybeckman.vn\/resources\/reading-material\/application-notes\/nanoscale-sorting-cytoflex-srt##\" data-featherlight=\"https:\/\/media.beckman.com\/-\/media\/flow-cytometry\/products\/instruments\/srt\/application-note\/nanoscale-sorting-cytoflex-srt-gating-strategy-3.jpg\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"center\" src=\"https:\/\/media.beckman.com\/-\/media\/flow-cytometry\/products\/instruments\/srt\/application-note\/nanoscale-sorting-cytoflex-srt-gating-strategy-3.jpg\" alt=\"CytoFLEX SRT Ph\u00e2n lo\u1ea1i c\u00e1c h\u1ea1t gi\u1ed1ng virus GFP+ v\u00e0 GFP-\" width=\"526\" height=\"566\" \/><\/a><figcaption class=\"wp-caption-text\">H\u00ecnh 4. Ph\u00e2n lo\u1ea1i c\u00e1c VLP tdTomato v\u00e0 GFP<\/figcaption><\/figure>\n<p><strong> (A)<\/strong>\u00a0M\u1ee9c t\u0103ng v\u00e0 ng\u01b0\u1ee1ng: VSSC 200, SSC 400, GFP 700, tdTomato 500. Ng\u01b0\u1ee1ng VSSC 4000.\u00a0<strong>(B)<\/strong>\u00a0M\u1ee9c t\u0103ng v\u00e0 ng\u01b0\u1ee1ng: VSSC 200, SSC 400, GFP 700, tdTomato 500. Ng\u01b0\u1ee1ng GFPH 1000 v\u00e0 tdTomato 1000.\u00a0<strong>( C)<\/strong>\u00a0S\u1ef1 ph\u1ee5c h\u1ed3i c\u1ee7a c\u00e1c qu\u1ea7n th\u1ec3 \u0111\u01b0\u1ee3c s\u1eafp x\u1ebfp d\u1ef1a tr\u00ean B. Ti\u1ebfng \u1ed3n m\u00e1y \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh b\u1eb1ng c\u00e1ch ch\u1ec9 ch\u1ea1y b\u1ed9 \u0111\u1ec7m m\u1eabu \u0111\u1ec3 cho ph\u00e9p \u0111i\u1ec1u ch\u1ec9nh ng\u01b0\u1ee1ng nh\u1eb1m lo\u1ea1i b\u1ecf ph\u1ea7n l\u1edbn ti\u1ebfng \u1ed3n.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Phan_ket_luan\"><\/span><strong style=\"font-size: 20.16px; color: #333333;\">Ph\u1ea7n k\u1ebft lu\u1eadn<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>M\u1ed9t s\u1ed1 k\u1ef9 thu\u1eadt \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e2n t\u00edch v\u00e0 m\u00f4 t\u1ea3 \u0111\u1eb7c t\u00ednh c\u1ee7a sinh h\u1ecdc c\u00f3 k\u00edch th\u01b0\u1edbc nano. Khi vi\u1ec7c s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p <a href=\"https:\/\/vospan.com\/product-category\/cong-nghe-sinh-hoc\/sinh-hoc-phan-tu\/may-dem-te-bao-dong-chay\/\">\u0111\u1ebfm t\u1ebf b\u00e0o d\u00f2ng ch\u1ea3y<\/a> trong l\u0129nh v\u1ef1c nghi\u00ean c\u1ee9u k\u00edch th\u01b0\u1edbc nano ng\u00e0y c\u00e0ng t\u0103ng v\u00e0 nhu c\u1ea7u ph\u00e2n l\u1eadp c\u00e1c h\u1ea1t c\u1ee5 th\u1ec3 ng\u00e0y c\u00e0ng c\u1ea5p thi\u1ebft, ch\u00fang t\u00f4i \u0111\u00e3 th\u1eed nghi\u1ec7m kh\u1ea3 n\u0103ng th\u1ef1c hi\u1ec7n ph\u00e2n lo\u1ea1i EV. Trong ghi ch\u00fa \u1ee9ng d\u1ee5ng n\u00e0y, ch\u00fang t\u00f4i s\u1ebd b\u00e1o c\u00e1o ph\u00e2n t\u00edch v\u00e0 ph\u00e2n lo\u1ea1i h\u1ea1t nano kh\u00e1c nhau b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng m\u00e1y ph\u00e2n lo\u1ea1i t\u1ebf b\u00e0o <a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-srt-may-phan-loai-te-bao-de-ban\/\">CytoFLEX SRT<\/a>. T\u1ed5ng h\u1ee3p d\u1eef li\u1ec7u \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y \u1edf \u0111\u00e2y cho th\u1ea5y r\u00f5 r\u00e0ng r\u1eb1ng m\u00e1y ph\u00e2n lo\u1ea1i t\u1ebf b\u00e0o<a href=\"https:\/\/vospan.com\/san-pham\/cytoflex-srt-may-phan-loai-te-bao-de-ban\/\"> CytoFLEX SRT <\/a>c\u00f3 kh\u1ea3 n\u0103ng ph\u00e2n lo\u1ea1i c\u00e1c h\u1ea1t c\u00f3 \u0111\u01b0\u1eddng k\u00ednh d\u01b0\u1edbi 1 micron. C\u00e1c th\u00ed nghi\u1ec7m nh\u1eb1m m\u1ee5c \u0111\u00edch ph\u00e2n lo\u1ea1i vi khu\u1ea9n, h\u1ea1t gi\u1ed1ng virus v\u00e0 h\u1ea1t \u0111\u00e3 t\u1ea1o ra k\u1ebft qu\u1ea3 c\u00f3 ch\u1ea5t l\u01b0\u1ee3ng cao. <a href=\"https:\/\/vospan.com\/product-brands\/beckman-coulter\/\">Beckman Coulter<\/a> c\u00f3 th\u1ec3 ch\u1ee9ng minh s\u1ef1 ph\u00e2n lo\u1ea1i c\u00f3 \u0111\u1ed9 tinh khi\u1ebft cao c\u1ee7a c\u00e1c h\u1ea1t c\u00f3 k\u00edch th\u01b0\u1edbc nano trong m\u1ed9t thi\u1ebft b\u1ecb c\u00f3 \u0111\u1ed9 ph\u1ee9c t\u1ea1p th\u1ea5p h\u01a1n \u0111\u00e1ng k\u1ec3 so v\u1edbi c\u00e1c gi\u1ea3i ph\u00e1p d\u1ef1a tr\u00ean ph\u01b0\u01a1ng ph\u00e1p <a href=\"https:\/\/vospan.com\/product-category\/cong-nghe-sinh-hoc\/sinh-hoc-phan-tu\/may-dem-te-bao-dong-chay\/\">\u0111\u1ebfm t\u1ebf b\u00e0o d\u00f2ng ch\u1ea3y<\/a> kh\u00e1c.<\/p>\n<p><em>Ngu\u1ed3n: <a href=\"https:\/\/www.mybeckman.vn\/resources\/reading-material\/application-notes\/nanoscale-sorting-cytoflex-srt\" target=\"_blank\" rel=\"noopener\">https:\/\/www.mybeckman.vn\/resources\/reading-material\/application-notes\/nanoscale-sorting-cytoflex-srt<\/a><\/em><\/p>\n<p><strong>Minh Khang l\u00e0 nh\u00e0 ph\u00e2n ph\u1ed1i v\u00e0 nh\u1eadp kh\u1ea9u tr\u1ef1c ti\u1ebfp c\u00e1c lo\u1ea1i <a href=\"https:\/\/vospan.com\/product-category\/cong-nghe-sinh-hoc\/sinh-hoc-phan-tu\/may-dem-te-bao-dong-chay\/\">m\u00e1y \u0111\u1ebfm t\u1ebf b\u00e0o d\u00f2ng ch\u1ea3y<\/a> h\u00e3ng<a href=\"https:\/\/vospan.com\/product-brands\/beckman-coulter\/\"> Beckman Coulter<\/a>.<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ebfm t\u1ebf b\u00e0o d\u00f2ng ch\u1ea3y \u0111\u1ec3\u00a0ph\u00e2n lo\u1ea1i h\u1ea1t nano l\u00e0 m\u1ed9t trong m\u1ed9t s\u1ed1 k\u1ef9 thu\u1eadt \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 m\u00f4 t\u1ea3 \u0111\u1eb7c t\u00ednh c\u1ee7a c\u00e1c h\u1ea1t si\u00eau nh\u1ecf. C\u00e1c h\u1ea1t sinh h\u1ecdc th\u01b0\u1eddng \u0111\u01b0\u1ee3c kh\u00e1m ph\u00e1 trong ph\u1ea1m vi k\u00edch th\u01b0\u1edbc n\u00e0y bao g\u1ed3m vi khu\u1ea9n, vi r\u00fat, t\u00fai ngo\u1ea1i b\u00e0o v\u00e0&#8230;<\/p>\n","protected":false},"author":3,"featured_media":10219,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[40],"tags":[],"class_list":["post-10218","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tin-tuc"],"acf":[],"_links":{"self":[{"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/posts\/10218"}],"collection":[{"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/comments?post=10218"}],"version-history":[{"count":6,"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/posts\/10218\/revisions"}],"predecessor-version":[{"id":11821,"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/posts\/10218\/revisions\/11821"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/media\/10219"}],"wp:attachment":[{"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/media?parent=10218"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/categories?post=10218"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vospan.com\/wp-json\/wp\/v2\/tags?post=10218"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}