- LVF Monochromators for wavelength flexibility and performance
- Automatic sensitivity adjustment with EDR
- Fast autofocus on the entire plate
- Optimized luminescence detection with automatic crosstalk reduction
- Scanning at absorbance, fluorescence and luminescence
- Dedicated features for live cell-based assays
- Comprehensive capabilities with top/bottom probes and auto switches
- Compact, space-saving stand
- Easy integration into automated systems
VANTAstar – Microplate reader
Flexible microplate reader with simplified workflow
The new VANTAstarTM is the latest addition. This compact multimode microplate reader is conceived for ease of use and flexibility, and ensures easy detection setup for simple data generation. Equipped with patented dual LVF Monochromators™ systems, filters, UV/vis spectrometers and Advanced Dynamic Range technology, it can cover a wide range of applications in basic research and science learn life.
Detection mode | Cuong +B1:B69uang (including FRET) |
Fluorescence polarization | |
Luminescent (flashes and glows) – BRET included | |
Time-resolved fluorescence | |
TR-FRET | |
UV/vis . absorption spectrum | |
Measurement mode | Read above and below |
Endpoints and kinetics | |
Sequential Multiple Stimulation | |
Sequential multicast | |
Scan spectrum (fluorescence, luminescence, absorbance) | |
Scale measurements | |
Sweep the well | |
Microplate form | 6-384 well plate, user definable |
LVis plate with 16 low-volume microspots (2 μL) | |
Microplate holder | Compatible with robots |
Light source | High-energy xenon flash. |
Probe | Low noise photomultiplier tube |
CCD . Spectrophotometer | |
Wavelength selection | Dual Linear Variable Filter (LVF) Monochromators™ |
Linear transform dichroic mirror | |
Separate monochromator LVF excitation and emission | |
Optical filter: | |
Excitation and emission sliders hold 5 filters on each side | |
LVF Monochromators + optical filter | |
Use one for excitation and the other for emission | |
UV/Vis Absorption Spectrometer: Full spectrum or 8 separate wavelengths for <1 second/well | |
Spectral band | Filter |
FI, LUM, TRF, FP, TR-FRET: 240-740 nm. | |
LVF Monochromators™ | |
FI, LUM: 320 – 740 nm. | |
Dichroic Linear Variation | |
340 – 740 nm. | |
Spectrum | |
ABS: 220 – 1000 nm; wavelength accuracy: 0.5 nm | |
Sensitivity | FI filter (top) |
<0.5 pM (<10 amol/FITC well, 384sv, 20 μL) | |
FI filter (bottom) | |
<2.5 pM (<125 amol/FITC well, 384g, 50 μL) | |
Monochrome FI (top) | |
<0.8 pM (<16 amol/FITC well, 384sv, 20 μL) | |
Monochrome FI (bottom) | |
<3.5 pM (<175 amol/FITC well, 384g, 50 μL) | |
Dynamic range: 8 decades in a single measurement | |
FP | |
<1.2 mP SD at 1 nM FITC (384 s, 20 μL) | |
HTRF® (black and white microplate) | |
Reader controller (Eu) after 18h (384sv, 20 μL) | |
Delta F > 700% (High Corrector) | |
Delta F > 25% (Low Corrector) | |
TRF | |
<30 fM europium (<2.4 amol/well, 384, 80 μL) | |
LUM | |
<0.8 pM (<15 amol/ATP well, 384sv, 20 μL) | |
Dynamic range: 8 decades in a single measurement | |
ABS with spectrometer | |
Selectable spectral resolutions: 1, 2, 5 and 10 nm | |
OD range: 0 to 4 OD; photometric resolution: 0.001 OD | |
Accuracy: <1% at 2 OD | |
Accuracy: <0.5% at 1 OD and <0.8% at 2 OD | |
Linearity: 0.8% at 2.0 OD | |
Reading time | Flight mode (flashes once) |
9 seconds (96), 16 seconds (384) | |
Shake | Linear, wobble and double wobble with user-defined time and speed |
INCUBATE | +4°C above ambient up to 45°C or 60°C |
Minimal condensation concept: the upper heating plate of the annealing chamber operates at +0.5°C above the lower plate, the optical system is heated | |
Software | Includes MARS Data Analysis Software and Intelligent Control for multiple users |
FDA 21 CFR Part 11 Compliant | |
Built-in fluorophore library | |
Size | Width: 35cm, depth: 52cm, height: 36cm; Weight: 27 kg |
Optical filter | Excitation and emission sliders up to 4 filters for monochromator (VANTAstar) and up to 5 filters per filter only (VANTAstar F). |
Optical path guide | Top and bottom: free-air optical light path guided by motor-driven and dichroic mirror |
Z-Adjustment | Auto focus height adjustment (0.1 mm resolution) |
Injection reagents | Up to 2 reagent injectors |
Individual injection volumes per well: 3 to 500 μL (up to 1 mL optional) | |
Variable spray speed up to 420 μL/s | |
Reagents are flushed | |
Heater and stirring plate | |
Size: width: 13cm, depth: 31cm, height: 26cm; Weight: 6 kg |
Plate Lvis | Sample capacity: 16 separate microdrop wells for 2 µL of sample; one standard cuvette position for up to 1 mL sample |
Internal standard quality control (optional): 04 NIST traceable optical density filters (approximate values 0.1, 0.3, 0.6 and 1.0 OD); a holmium oxide filter for wavelength accuracy. | |
Size: conforms to SBS standards for microsamples. | |
THERMOSSTAR | Microplate incubators and shakers |
Air Controller (ACU) | Actively regulate O2 and CO2 from 1-20% |
Optical filter | Excitation and emission filters |
Upgrade | Upgrades are available to include options like additional detection modes, reagent nozzles, extended temperature control, etc. |
- Quantification of DNA, RNA and proteins
- ELISA and DELFIA immunoassays
- Bacterial Growth (OD600)
- Cell viability/toxicity
- Real-time cell-based tests
- Enzyme activity
- Reporter gene testing…