The Brilliant II qRT-PCR core reagent kit includes the components necessary to carry out cDNA synthesis and PCR amplification in one tube and one buffer. Brilliant kits support quantitative amplification and detection with multiplex capability and show consistent high performance with various fluorescent detection systems, including molecular beacons and TaqMan probes. The one-step core reagent kit successfully amplifies and detects high- and low-abundance RNA targets, from experimental samples including total RNA, poly(A)+, and synthetic RNA.