The Brilliant II qPCR probe master kit with ROX includes the components necessary to carry out PCR amplification in one tube and one buffer. Brilliant II kits support quantitative amplification and detection with multiplex capability and show consistent high performance with various fluorescent detection systems, including molecular beacons and TaqMan probes. Amplify and successfully detect high- and low-abundance RNA targets, from experimental samples including total RNA, poly(A)+, and synthetic RNA.