The most versatile plate reader for test development
CLARIOstar® Plus is the most versatile multi-mode plate reader. It is equipped with patented LVF Monochromators™, filters and spectrometers. Flexibility combined with best-in-class sensitivity and newly developed Advanced Dynamic Range technology, make it the ideal reader for test development.
| Detection mode | Fluorescence intensity (including FRET) |
| Fluorescence polarization | |
| AlphaScreen® / AlphaLISA® / AlphaPlex™ | |
| Luminescent (flashes and glows) – BRET included | |
| Time-resolved fluorescence | |
| TR-FRET | |
| UV/vis . absorption spectrum | |
| Measurement mode | Read above and below |
| Endpoints and kinetics | |
| Sequential Multiple Stimulation | |
| Sequential multicast | |
| Scan spectrum (fluorescence, luminescence, absorbance) | |
| Scale measurements | |
| Sweep the well | |
| Microplate form | 6 – 1536 wells, user definable |
| LVis plate with 16 low-volume microspots (2 μL). | |
| Microplate holder | Compatible with robots |
| Light source | High-energy xenon flash |
| Specialized lasers for AlphaScreen® / AlphaLISA® / AlphaPlex™. | |
| Probe | The photomultiplier tube has low noise. |
| Red-shifted photomultiplier tube | |
| CCD Spectrophotometer | |
| Wavelength selection | Monochromators™ Dual Linear Transform Filters (LVFs). |
| Linear transform dichroic mirror. | |
| Separate mono set LVF ex & em. | |
| Optical filter: | |
| ex & em sliders hold 4 filters on each side | |
| LVF Monochromators + optical filter | |
| Use one for ex and the other for em | |
| UV/Vis Absorption Spectrophotometer: | |
| Full spectrum or 8 separate wavelengths in <1 second/well | |
| Spectral band | Filter |
| FI, FP, TRF: 240-740 nm or 240-900 nm (red-shifted PMT) | |
| LUM: 240 – 740 nm | |
| LVF Monochromators™ | |
| FI: 320 – 740 nm or 320 – 840 nm (red-shifted PMT) | |
| LUM: 320 – 740 nm | |
| Linear Variation Dichroism | |
| 340 – 740 nm or 340 – 760 nm (red-shifted PMT) | |
| Spectrum | |
| ABS: 220 – 1000 nm; wavelength accuracy: 0.5 nm | |
| Sensitivity | FI filter (top) |
| <0.15 pM (<3 amol/FITC well, 384sv, 20 μL) | |
| FI filter (bottom) | |
| <1.0 pM (<50 amol/FITC well, 384g, 50 μL) | |
| Monochrome FI (top) | |
| <0.35 pM (<7 amol/FITC well, 384sv, 20 μL) | |
| Monochrome FI (bottom) | |
| <3.0 pM (<150 amol/FITC well, 384g, 50 μL) | |
| Dynamic range: 8 decades in a single measurement | |
| FP | |
| <0.5 mP SD at 1 nM FITC (384 s, 20 μL) | |
| HTRF® (black and white microplate) | |
| Reader controller (Eu) after 18h (384sv, 20 μL) | |
| Delta F > 880% (High Corrector) | |
| Delta F > 30% (Low Corrector) | |
| TRF | |
| <20 fM europium, 384, 80 μL | |
| LUM | |
| <0.4 pM (<8 amol/ATP well, 384sv, 20 μL) | |
| Dynamic range: 8 decades in a single measurement | |
| AlphaScreen® with laser | |
| <5 pM (<100 amol/well P-Tyr-100, 384sv, 20 μL) | |
| ABS with spectrometer | |
| Selectable spectral resolutions: 1, 2, 5 and 10 nm | |
| OD range: 0 to 4 OD; photometric resolution: 0.001 OD | |
| Accuracy: <1% at 2 OD | |
| Accuracy: <0.5% at 1 OD and <0.8% at 2 OD | |
| Linearity: 0.8% at 2.0 OD | |
| Reading time | Flight mode (flashes once) |
| 8 seconds (96), 15 seconds (384), 28 seconds (1536) | |
| 10 flashes | |
| 19 seconds (96), 57 seconds (384), 3 minutes 4 seconds (1536) | |
| Shake | Linear, orbital and dual trajectories with user-defined time and speed. |
| INCUBATE | +3°C above ambient up to 45°C or 65°C |
| The upper heating plate of the annealing chamber operates at a temperature 0.5°C higher than the lower plate. This prevents steam build-up on the lid or sealing gasket. | |
| Software | Includes multi-user reader control software and MARS data analysis software. |
| FDA 21 CFR Part 11 Compliant | |
| Integrated fluorophore library |
| Plate Lvis | Sample capacity: 16 separate microdrop wells for 2 µL of sample; one standard cuvette position for up to 1 mL sample |
| Internal standard quality control (optional): 4 NIST traceable optical density filters (approximate values 0.1, 0.3, 0.6 and 1.0 OD); a holmium oxide filter for wavelength accuracy | |
| Size: conforms to SBS standards for microsamples | |
| THERMOSSTAR | Microplate incubators and shakers |
| Air Controller (ACU) | Actively regulate O2 and CO2 from 0.1-20%. |
| Stacker | Can hold up to 50 plates, providing continuous loading capacity |
| Optical filter | Excitation and emission filters |
| Upgrade | CLARIOstar Plus may include all or any combination of the features listed below upon purchase. Upgradeable with additional features at any time. |
Microplate Reader
Microplate Reader
Microplate Reader
Microplate Reader
Microplate Reader
Microplate Reader
Microplate Reader
Microplate Reader
UV/vis SPECTROstar Omega – Absorption plate reader with spectrometer
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