Công ty TNHH TM DV KT Minh Khang //vospan.com Thu, 05 Oct 2023 01:38:40 +0000 en-US hourly 1 //wordpress.org/?v=6.1.4 //vospan.com/wp-content/uploads/2021/02/favicon-32x32.png Công ty TNHH TM DV KT Minh Khang //vospan.com 32 32 Công ty TNHH TM DV KT Minh Khang //vospan.com/en/mou-signing-ceremony-pathology-laboratory-of-leica-biosystems-equipment/ Thu, 05 Oct 2023 01:18:35 +0000 //vospan.com/?p=9811

With the desire to contribute a part in the development of the field of Pathology as well as the mission of serving the community. On the morning of October 4, 2023, Applied Health Innovation Co., Ltd. (a member company of Minh Khang Technical Trading Service Co., Ltd.) signed a memorandum of cooperation (MOU) with the University of Medicine and Pharmacy, VNU established the Pathology Lab at the school.

This demo lab not only supports Minh Khang’s customers to visit, experience Leica Biosystems’ most modern analysis/pathology equipment; but also used in new research topics, as well as performing training functions, which helps to improve analysis/ testing/ diagnosis capacity in the field of pathology in Vietnam.

Congratulations for this successful cooperation, as well as wishes for faster and stronger development of the field of pathology, contributing to cancer screening and prevention in Vietnam.
#UMPVNU #MinhKhang #AHI #MOU #LeicaBiosystems
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Minh Khang Company is the exclusive partner of Leica Biosystems, pathology equipment

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Công ty TNHH TM DV KT Minh Khang //vospan.com/en/automated-1-hour-mycoplasma-testing-in-bioproduction/ Mon, 15 May 2023 02:55:15 +0000 //vospan.com/?p=8043

INTRODUCTION

Various pharmacopoeias, including the USP, specify a test for mycoplasma  contamination must be performed as part of the release testing of a product manufactured in the presence of eukaryotic cells (1).
Current compendial methods require ?8 days to generate results creating delays  releasing product to downstream processes. Alternative conventional nucleic acid testing (NAT) methods are available; however, until recently these methods provided speed (~5 h) but not ease of use.

The BIOFIRE® FILMARRAY® 2.0 industry system utilizes a film array instrument and next generation PCR testing where all reagent components are contained in a closed pouch to detect the presence of >130 species of mycoplasma. The system provides sample to answer in ~1 hour with little technical training  needed providing options for bringing mycoplasma testing directly to the production floor where critical testing is needed.

BIREFIRE testing chart

*10 mL protocol

As presented at the 2021 PDA Pharmaceutical Microbiology Virtual Conference, the data summarized in this White Paper is from four bioproduction manufacturers that evaluated the BIOFIRE®FILMARRAY® 2.0 Industry system (2). Samples were evaluated with up to 9 compendial mycoplasma species in the presence of high-density monoclonal antibody producing Chinese hamster ovary (CHO) cells. Studies were designed to assess product interference (false-positive rates), and detection (false-negative rates) including level of detection (LOD). Two distinct protocols were evaluated: a protocol using 10 mL of product sample that provides the sensitivity for release testing, and a direct-test protocol using 0.2 mL product sample that allows for at-line in-process control testing.

THE VALUE OF RAPID, EASY MYCOPLASMA TESTING

A faster, easier approach to mycoplasma testing can bring quantifiable value to bioproduction manufacturers. From simplifying training requirements, to reducing investigations linked to human error, to providing an early alert to contamination, an ultra rapid, ultra easy mycoplasma test can save time and money bringing value to any bioproduction organization.
The BIOFIRE® mycoplasma test can be performed by anyone, anywhere at anytime allowing manufacturers to take critical quality testing out of the lab and closer to manufacturing to realize the following benefits:

?Simplified training requirements
?Lower expertise required
?Objective results
?Less human error risk/improved Data Integrity
?Does not require PCR lab
?1 hour time-to-result brings flexibility in testing planning
?Provides early alert to contamination and reduces costs of non-quality

anyone, anywhere, anytime

Because BIOFIRE® FILMARRAY® 2.0 is so easy to use, manufacturers who previously lacked the resources to perform mycoplasma testing in-house can now easily perform mycoplasma screening at-line with little specialized equipment or training saving on costly outsourced laboratory testing.

Bioproduction manufacturers today waiting hours or weeks for results using complex testing methods can realize significant value with a more rapid, simplified approach to mycoplasma testing using BIOFIRE®FILMARRAY® 2.0 Industry.

<60 min chart

BIOFIRE FILMARRAY 2.0 INDUSTRY SYSTEM

The system utilizes a FILMARRAY® 2.0 Industry instrument and next generation PCR testing in a closed pouch to detect the presence of mycoplasma (Figures 1 and 2). The disposable BIOFIRE® Mycoplasma pouch contains all of the necessary reagents for automated cell lysis, nucleic acid purification, reverse transcription, first and second stage nested PCR and analyst detection in order to isolate, amplify and detect over 130 different mycoplasma species (Figure 2).

Several controls are integrated into the pouch to ensure the quality of the results including a total process control, reverse transcription control, and PCR I and PCR II controls. The instrument and software process the pouch with results in less than an hour.

The FILMARRAY® 2.0 Industry software (21 CFR Part 11 compliance ready) performs all of the complex meta-analysis and provides presence/absence results as either “Mycoplasma Detected” or “Mycoplasma Not Detected?

Figure 1:

FILMARRAY® 2.0 Industry instrument performs the extraction, amplification and detection (25.4 x 39.3 x 16.5 cm WxDxH). The system comes standard with 2 instruments; up to 8 instruments can be connected to a single PC.

FILMARRAY
BIOFIRE MYCOPLASMA

Figure 2:

A. BIOFIRE® Mycoplasma pouch.

B. Pouch diagram: (A) Fitment with freezedried reagents; (B) Plungers-deliver reagents to blisters; (C) Sample lysis and bead collection; (D) Wash; (E) Magnetic bead collection blister; (F) Elution; (G) Multiplex Outer PCR blister; (H) Dilution blister; (I) Inner Nested PCR array

SAMPLE PROTOCOLS

Two distinct protocols have been designed to detect mycoplasma contamination(3). These include a 0.2 mL direct test that can be used for in-process control testing with a validated LOD of ~?0 CFU/ mL, and a 10 mL release test that concentrates the sample using centrifugation and has a validated LOD of ?0 CFU/mL.

These protocols were followed by evaluators except that Evaluator B deviated from the manufacturer recommendations with customized centrifugation times and forces. Following sample pre-processing, samples were then loaded onto a fully prepared and hydrated pouch and run on the FILMARRAY® 2.0 Industry instrument.

sample protocol

Source: //www.biomerieux.com/corp/en/resource-hub/knowledge/white-papers/pharmaceutical-qc-white-papers/automated-1-hour-mycoplasma-testing-in-bioproduction.html

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Công ty TNHH TM DV KT Minh Khang //vospan.com/en/leicas-4-tips-for-digital-pathology-slide-scanning/ Mon, 06 Mar 2023 08:05:47 +0000 //vospan.com/?p=7633

Do you want to get better digital images from your glass slides? Here are some tips to help you get the best out of your digital pathology line scanner:

TIP 1: Tissue Preparation

It’s vital to have well-prepared tissue ?the best digital scanner can’t compensate for poor samples. Here are some aspects of tissue preparation that can affect the digitization process.

Cutting ?Digital pathology scanners have a smaller range of focus than a traditional microscope, so keep this in mind. When scanning single-plane images, standard tissue thickness (3 ?5 μm) will give best results. For thick sections, multi-plane (z-stack) scanning may be necessary.
Staining ?Very faint staining or excessive background staining will make it more difficult for the scanner to automatically identify the tissue, and may mean more manual work.
Mounting ?As with traditional microscopy, digital pathology scanners work best on flat tissue surfaces. Avoid folds or wrinkles in the tissue for optimal focus.
Coverslipping ?Glass coverslips are preferable if you plan to scan the slide, as plastic coverslips may warp over time and affect scanner focus. As with traditional microscopy, ensure that no air bubbles are trapped under the coverslip, to prevent areas being scanned out of focus.
TIP 2: Slide Inspection & Preparation

To improve results with your slides, always check and clean them before scanning.

Inspect slides for overhanging labels, tape, or excess mounting medium that may affect the fit of the slide in the scanning mechanism and impede operation.
Carefully wipe slides down with a soft cloth (e.g. microfiber) to remove loose debris, water spots, or fingerprints from the upper and lower surface. For difficult stains, dampen the cloth with water or alcohol solution to clean.
Check for cracks or chips in the slide, particularly at the corners. Any loose glass shards could fall off during scanning and damage the scanning mechanism.
Wait until slides are fully dry before loading into the scanner, to avoid residue getting on the scanner mechanism.
Ensure slides are placed flat into the scanner rack or carrier, and any securing mechanism engaged. If the slide is not flush during scanning, there is a high likelihood of unfocused areas in your scan. Labels on the bottom of the slide may prevent the slide flaying flat, so ensure these are only placed on the top-facing side of the slide.
TIP 3: Scanning your Slide

Each digital pathology scanner model has its own workflow and requirements. However, there are some general guidelines to follow in order to get a good scan.

The scanner should automatically detect all the tissue on the slide, while excluding non-relevant objects such as labels, pen marks, or debris. However faint tissue staining, excessive background staining, mounting media discoloration, or excessive debris / marks on slides can lead to poor tissue detection. If possible, review the tissue detection on suboptimal slides prior to high resolution scanning, to avoid the need for rescans later.
Focusing should be automatic, and focus points ?if present ?should be evenly distributed over the tissue. If areas of the slide are not scanning in focus, try placing additional focus points manually.
Avoid placing focus points on areas where there is debris on / under the coverslip, air bubbles, or other defects. These may cause the scanner to focus on the incorrect scanning plane, leading to out-of-focus tissue.
For oil scanning at higher magnifications, ensure that enough oil is applied before scanning to prevent the objective drying out during the scanning process, which can leave “streaking?artifacts on the scanned image. However too much oil can seep under the cover slip, leaving a “glazed?appearance on the scan with poor contrast along cell edges. Test scans are a good practice with oil scanning. Be sure to clean up any oil residue on the racks or scanning mechanism after scanning.
TIP 4: Quality Control (QC)
Despite all your efforts, not every scan will be acceptable first time. QC processes will vary per lab, and you should always follow the QC requirements of your institution. However here are some steps you can follow for quick and efficient scan QC in your image viewer.
Review the whole slide ?or at least the entire scanned tissue area ?at a low magnification (e.g. 4x), checking for visible out-of-focus areas or misalignments. Misalignments will typically be visible as a “stitch line?through the image, where the scanned stripes that make up the full image meet.
Zoom into the magnification at which the high resolution image was captured, and review in a single horizontal path across the tissue section at its widest point, one field of view at a time. This will allow you to check for smaller areas of poor focus or subtler misalignments.
Perform the same field-by-field review in the vertical direction, from one edge of the tissue to the other in an unbroken line, checking again for any out-of-focus areas.
Spot check a few areas in the slide at high magnification, particularly any regions where the tissue is a different thickness or where there are any defects in the slide. These are the areas most likely to have poor focus.

Follow these simple guidelines when scanning, to help you produce higher quality digital images and give you a permanent record of your slides that you can view, share, and analyze.

Source: //www.leicabiosystems.com/clinical-solutions/resources/4-tips-for-digital-pathology-slide-scanning/

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Công ty TNHH TM DV KT Minh Khang //vospan.com/en/echo-acoustic-technology/ Thu, 02 Mar 2023 03:17:24 +0000 //vospan.com/?p=7582 MOVING LIQUIDS with SOUND

Delivering Precise, Accurate, and Cost-efficient Liquid Transfers

The Echo Liquid Handler has revolutionized liquid handling with an innovative technology that produces faster and more accurate results than traditional pipetting methods. Whether you are involved in drug discovery, compound management, genomics research, synthetic biology, proteomics, functional screening, or other research applications, you can do better science and save money with the Echo acoustic droplet ejection (ADE) technology.

Powerful but gentle in its approach, ADE drives Echo Liquid Handlers. It focuses ultrasonic acoustic energy at the meniscus of a fluid sample to eject small droplets of liquid from wells and position them precisely onto a surface suspended above the ejection point.

Echo Acoustic Liquid Handling

The Echo Liquid Handler revolutionizes liquid handling by using acoustic energy. Sound waves eject precisely sized droplets from a source onto a microplate, slide or other surface suspended above the source. The Echo Liquid Handler can transfer as many as 750,000 samples a day to 384-, 1536-, and 3456-well plates, slides, and microfluidic devices, without touching the samples. This groundbreaking technology benefits laboratory workflows and operations of all sizes.

DYNAMIC FLUID ANALYSIS

On-the-Fly Adjustments to Changing Fluid Properties

Dynamic Fluid Analysis enables Echo Liquid Handlers to adapt easily to different types of fluids. This patented software algorithm:

  • Adjusts acoustic energy requirements in real time without operator intervention
  • Eliminates manual calibration
  • Ensures accurate, precise fluid transfers
  • Increases the range of fluids transferred by acoustic energy

The Echo Liquid Handler uses Dynamic Fluid Analysis capabilities to simplify experimental setup and enables a higher degree of experimental and workflow flexibility.

Key Benefits

  • No need to calibrate the instrument in advance ?the Echo Liquid Handler determines transfer parameters for each reagent at runtime.
  • Larger-volume transfers are accomplished by transferring the same drop size repeatedly—there’s no requirement to scale with volume.
  • Dynamic Fluid Analysis is an active process—and therefore less sensitive to reagent changes than a single calibration point—so downstream experimental data can be more consistent.
  • Dynamic Fluid Analysis determines the appropriate transfer parameters on a well-by-well basis, and thus works with more complex reagent sets or inconsistent reagents.

Background on Fluid Transfer and Liquid Handlers

Fluid transfer methods are impacted by two key fluid properties: surface tension (the way fluid at an interface interacts with its surroundings) and viscosity (the resistance of fluid to move). Most liquid handlers are passive and require operators to determine how to overcome surface tension and viscosity for their specific reagents, putting the burden on them to calibrate the instrument to transfer that reagent.

This burden is substantial, because calibrations:

  • Must be verified and repeated on every instrument that is required to transfer that reagent.
  • Usually work over only a limited volume range.
  • May drift with the age of the liquid handler as mechanical components wear down.
  • Are only an approximation; calibrations against inconsistent reagents (e.g., cell lysates in a screen, or high-viscosity reagents) may not transfer accurately and precisely, which may lead to data errors downstream.
  • Cannot be developed for certain types of reagents on multichannel liquid handlers (e.g., commercially available crystallography reagents, by design, consist of reagents of varying viscosity and surface tension properties).

Dynamic Fluid Analysis Technology Improves Liquid Handling

Echo Liquid Handlers use sound energy to transfer reagents in drop increments on a 2.5 or 25 nL scale (instrument dependent). All models incorporate a transducer, which converts electrical energy to sound energy to accomplish the drop transfer. The transfer process can be explained in two broad steps:

Dynamic fluid analysis, Echo acoustic technology

SURVEY: The Echo Liquid Handler determines fluid height and properties in the source wells. The transducer (acoustic energy generator) sends soft energy pings into each well and receives the reflections (or echoes) returned from three interfaces:

  • The bottom of the source microplate
  • The bottom of the well
  • The fluid meniscus

TRANSFER: The transducer returns to the first transfer point and sends a larger energy burst to accomplish the transfer in discrete, consistent droplets. Key to this process is that the transducer interfaces with one well at a time. This affords the capability to perfect transfer for each source well. General information on the fluid type provided by the operator (e.g., DMSO, buffer, reagent containing glycerol, etc.) narrows the transfer parameters.

Dynamic Fluid Analysis uses a power-adjustment ramping process to slowly increase the energy from survey power level to transfer power levels. By listening to subsequent echoes, the Echo Liquid Handler can determine the appropriate power level required for transfer from each source well. This process is accomplished in milliseconds and requires no operator intervention.

Source: //www.mybeckman.vn/liquid-handlers/echo-acoustic-technology

Our products: //vospan.com/en/product-category/biological-technology/biological-analysis-instrument/automated-sample-preparation/acoustic-liquid-handlers/

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Công ty TNHH TM DV KT Minh Khang //vospan.com/en/microscopy-solutions-for-digital-classrooms-carl-zeiss-x-minh-khang/ Tue, 21 Feb 2023 04:09:02 +0000 //vospan.com/?p=7559 As science and technology are developing nowadays, digital transformation in education is a very popular method.

The Digital Classroom solution with smart microscopes from ZEISS provides the foundation for implementing digital interactive classrooms, providing an intuitive teaching and learning experience, which helps to promote the passion for scientific discovery among students.

The Digital Classroom solution is a Wi-Fi enabled microscope system that can connect to Instructors and students’ iPads, iPhones or Windows-PCs, allowing Instructors to use document sharing features, slideshows and many other useful tools to make practical lessons more interesting and engaging than ever.

Minh Khang Co., Ltd will organize a completely free Demo Workshop series to introduce the “Digital Classroom Solution” with the ZEISS microscope system.

If you are interested in this solution, please contact Minh Khang Company for advice and support, or leave information in the following form:

//forms.gle/46KddwpjLWR8WGpM7

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Công ty TNHH TM DV KT Minh Khang //vospan.com/en/minh-khang-exclusive-distributor-of-leica-biosystems-in-the-south-of-vietnam/ Fri, 03 Feb 2023 08:52:21 +0000 //vospan.com/?p=7204 Minh Khang Technical Trading & Service Co., Ltd officially became the distributor of Leica Biosystems in the South region – Vietnam market!

Leica Biosystems, founded in 1989, is one of the world’s leading companies providing comprehensive solutions for instruments, chemicals and consumables in pathology, from biopsy to diagnosis. .

Leica Biosystems is a member of the Danaher group, with headquarters in Germany and operations in more than 100 countries. The mission of “Enhancing cancer diagnosis, improving lives?is at the heart of the development of Leica Biosystems.

This is our honor to become the exclusive distributor in the South of Vietnam of Leica Biosystems!

 

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Công ty TNHH TM DV KT Minh Khang //vospan.com/en/minh-khang-exclusive-distributor-of-zeiss-microscopes-in-the-south-of-vietnam/ Fri, 11 Nov 2022 07:05:00 +0000 //vospan.com/?p=6870
📣On the morning of November 9, 2022, Minh Khang Trading and Technical Services Co., Ltd. had a signing ceremony with the representative of ZEISS Microscopy (Germany) to officially become the exclusive distributor in the South of Vietnam, microscope segment.
👉As the leading microscope manufacturer in Germany, Zeiss offers innovative solutions and services for research, education and clinical analysis. Zeiss instruments are trusted for high-tech manufacturing, assembly industries as well as materials research.
👉The signing ceremony took place successfully with the joy of both parties, with the common goal is bringing together the most comprehensive and quality products and services to customers.
We sincerely thank Zeiss for the cooperation and trust in Minh Khang and we hope that this relationship will reap many brilliant achievements in the future.
#CarlZeiss #microscopes #MinhKhang
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Công ty TNHH TM DV KT Minh Khang //vospan.com/en/growth-promotion-testing-of-selective-media/ Fri, 28 May 2021 03:28:35 +0000 //vospan.com/?p=4518 Growth promotion testing (GPT) of selective media following Microbiological Examination of Non- Sterile Products: Tests for Specified Microorganisms Ph.Eur.2.6.13 / USP<62> / JP 4.05 can be quite challenging. Achieving proficiency in selective media GPT requires a strong understanding of the pharmacopeia guidelines and the role of selective media.

Selective media are designed to provide a conducive environment for growth of some types of microorganisms but not to others. They provide the ability to control what will and will not grow on a culture plate. However, it is important to note that a microorganism will not grow as well on a selective media compared to a non-selective media. This is entirely due to non-selective media being well balanced with all the elements that most bacteria need to grow and flourish, whereas in selective media, due to the presence of either activating or inhibiting elements, selected microorganism will grow but not thrive.

Therefore, compendial test requirements for selective media are not quantitatively evaluated. Instead, pharmacopeia requirements for selective media testing state:

Growth of the microorganism comparable to that previously obtained with a previously tested and approved batch of medium occurs.

No percent recovery requirement must be achieved when testing a selective media batch. The qualification is based on growth of the new batch of selective media comparable to growth of previously tested and approved batch of the same selective media.

Source: Biomerieux

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